Researchers Identify Stable Reference Genes for qRT-PCR Analysis in Corydalis saxicola Under Abiotic Stress

Researchers have identified and validated a set of stable reference genes for quantitative real-time PCR (qRT-PCR) analysis in *Corydalis saxicola* subjected to various abiotic stress conditions. The study establishes a reliable framework for normalizing gene expression data, providing a standardized method for future molecular research on this medicinal plant species.

The research team evaluated the expression stability of candidate reference genes under multiple environmental stressors, including drought, salinity, heat, and cold. By utilizing statistical algorithms such as geNorm, NormFinder, and BestKeeper, the investigators determined which genes maintained the most consistent expression levels across different experimental treatments. The findings indicate that specific gene combinations provide the most accurate normalization for transcriptomic studies in *C. saxicola*. This validation process addresses the need for consistent internal controls, as the accuracy of qRT-PCR results depends heavily on the stability of reference genes under changing environmental variables.

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Source: GO-AI-ne1

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Date: June 3, 2026

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GOAI